Rietdorf J. (ed.) Microscopy Techniques

Springer, 2005. — 322 p.Advances in microscopy techniques have recently had a tremendous impact on research in biochemistry and molecular biology.While the study of bio chemical reactions was formerly confined to cuvette-based measurements of purified biomolecules, it is now possible for investigators to make such analyses inside the complex environment of living cells and organisms.
While some of these advanced techniques were in fact developed some time ago, it is only now that they are becoming available to a wider range of researchers as components of commercial equipment.As a consequence, there is a strong demand for the ability to help individuals from a biomedical, rather than from an instrumentation background, in order for them to make the best use of these new tools in their research. To meet these requirements, the contributions in this book mainly stem from instructors present at a series of very successful training courses held in the Advanced Light Microscopy Facility at the European Molecular Biology Laboratory over the past 5 years.Engineering Fluorescent Proteins
Higher Harmonic Generation Microscopy
Live Cell Spinning Disk Microscopy
Total Internal Reflection Fluorescence Microscopy in Single Molecule Nanobioscience
Fluorescence Correlation Spectroscopy with Autofluorescent Proteins
Fluorescence Recovery After Photobleaching: Application to Nuclear Proteins
Deconvolution Microscopy
Spectral Imaging and Linear Unmixing in Light Microscopy
Tracking Movement in Cell Biology