Gerwig G.J. The Art of Carbohydrate Analysis

Springer International Publishing, 2021. — 362 p.The growing importance of glycobiology and carbohydrate chemistry in modern biotechnology and the pharmaceutical industry makes accurate carbohydrate analysis indispensable. This book provides the principles and protocols of various fundamental carbohydrate analysis methods. Choice of method is entirely dependent upon the type of material being investigated (biological samples, food products, etc.), and the level of structural detail required, i.e. sugar content, compositional analysis, linkages between the sugar components, or the total chemical structure of a given molecule. Full structural characterization of carbohydrate chains requires significant time, resources, and skill in several methods of analysis; no single technique can address all glycan analysis needs.
This book summarizes several existing analytical techniques (both chemical and physical) in an introductory volume designed for the non-expert researcher or novice scientist. While background in carbohydrate chemistry is assumed, all information necessary to understanding the described techniques is addressed in the text.Preface
Acknowledgments
Disclaimer
About the Author
Abbreviations
The World of Carbohydrates
Basic Knowledge of Glycobiology
Carbohydrates Involved in Diseases
Detection of Carbohydrates by Colorimetric Methods
Analytical Techniques to Study Carbohydrates
Monosaccharide Composition Analysis
Carbohydrate Analysis of Glycoconjugates
Structural Characterization of Released Glycans
Analysis of Sialic Acids
Carbohydrate Microarray Technology
Analysis of Carbohydrates by Mass Spectrometry
Analysis of Carbohydrates by Nuclear Magnetic Resonance Spectroscopy
Glycobioinformatics
Concluding Remarks
Glossary
Index