Freitag R. (ed.) Modern advances in chromatography

Advances in Biochemical Engineering/ Biotechnology, Vol.
76. Managing Editor: Th. Scheper. Springer-Verlag Berlin Heidelberg, 2002. - 255 p.Chromatography is the separation method for biological molecules from the analytical to the preparative scale. Few of the recent advances in life science, whether in "proteomics" or in the industrial production of recombinant therapeutics, would have been possible without the help of chromatographic separations. Concomitantly, chromatography nowadays stands for much more than a batch column packed with porous particles. In this book eminent experts from academia and industry introduce the reader to some of the recent new developments in this exciting area. Ranging from evolving nanoanalytical techniques to advances in the material sciences and artificial antibodies and finally continuous large scale separations of the most fragile biologicals, this book should provide interesting reading material for students and practitioners from various fields.Due to their versatility and resolution, chromatographic separations of complex mixtures of biologicals are used for many purposes in academia and industry. If anything, recent developments in the life sciences have increased the interest and need for chromatography be it for quality control, proteomics or the downstream processing of the high value products of modern biotechnology. However, the many "challenges"of present day chromatography and especially of the HPLC of biomacromolecules such as proteins, are also present in the mind of any practitioner. In fact, some of these latter were such hindrances that much research was necessary in order to overcome and circumvent them. This book introduces the reader to some of the recently proposed solutions. Capillary elec-trochromatography (CEC),for example,the latest and most promising branch of analytical chromatography, is still hindered from finding broader application by difficulties related to something as simple as the packing of a suitable column.Capillary Electrochromatography: A Rapidly Emerging Separation Method
Short Monolithic Columns as Stationary Phases for Biochromatography
Porous Polymer Monoliths: An Alternative to Classical Beads
Molecularly Imprinted Materials – Receptors More Durable than Nature Can Provide
Chromatographic Reactors Based on Biological Activity
Simulated Moving Bed Chromatography (SMB) for Application in Bioseparation
Continuous Annular Chromatography